Biologists Discover How Salt Stops Plant Growth

It has not been clear how salt halts the growth of the plant-root system, until now. According to an international study published in the journal Plant Cell, an inner layer of tissue in the branching roots that anchor the plant is sensitive to salt and activates a stress hormone, which stops root growth.

This is a microscope image of a branching root, the cell boundaries are in red and the GFP fluorescent signal marks the endodermis (José Dinneny)

Salt accumulates in irrigated soils due to the evaporation of water, which leaves salt behind. Roots are intimately associated with their environment and develop highly intricate branched networks that enable them to explore the soil. The branching roots grow horizontally off the main root and are important for water and nutrient uptake.

“An important missing piece of the puzzle to understanding how plants cope with stressful environments is knowing when and where stressors act to affect growth,” said study senior author Dr José Dinneny of Carnegie Institution for Science.

The biologists grew seedlings of a laboratory plant (Arabidopsis) that is a relative of mustard using a custom imaging system, which enabled them to measure the dynamic process of root growth throughout the salt response.

This ability to track root growth in real time led the researchers to observe that branching roots entered a dormant phase of growth as salt was introduced. To determine how dormancy might be regulated, they surveyed the role of different plant hormones in this process and found that a critical plant hormone called abscisic acid was the key signaling molecule.

“We are familiar with how animals use a fight or flight strategy to face external challenges. While plants can’t run for safety, they can control how much they grow into dangerous territory. It turns out that abscisic acid, a stress hormone produced in the plant when it is exposed to drought or salty environments, is important in controlling the plant equivalent of fight or flight,” Dr Dinneny said.

To understand how Abscisic Acid controls growth, the biologists devised a strategy to inhibit the response to this hormone in different tissue layers of the root. They developed several mutants in which the response to the hormone was suppressed in different root layers. They found that a significant portion of the salt response was dependent upon how a single cell layer sensed the hormone. The live imaging allowed them to watch what happened to root growth in these mutant plants.

Lead author Dr Lina Duan, also of Carnegie Institution for Science, said: “Interestingly, the ‘inner-skin’ of the root, called the endodermis, was most critical for this process. This tissue layer is particularly important as it acts like a semipermeable barrier limiting which substances can enter the root system from the soil environment.”

“Our results mean that in addition to acting as a filter for substances in the soil, the endodermis also acts as a guard, with abscisic acid, to prevent a plant from growing in dangerous environments,” Dr Dinneny concluded. “Irrigation of agricultural land is a major contributor to soil salinity. And as sea levels rise with climate change, understanding how plants, particularly crops, react to salt might allow us to develop plant varieties that can grow in the saltier soils that will likely occur in coastal zones.”

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Bibliographic information: Duana L et al. Endodermal ABA Signaling Promotes Lateral Root Quiescence during Salt Stress in Arabidopsis Seedlings. The Plant Cell, published online before print January 2013; doi: 10.1105/tpc.112.107227