Researchers Identify Taste Stem Cells

Neurobiologists from the United States and Japan have identified the location and genetic characteristics of taste stem cells on the tongue.

An image showing that the strongest-staining Lgr5-GFP cells (green) are adjacent to the opening of the duct of Von Ebner’s gland below the circumvallate papilla, type II taste receptor cells were identified by anti-Trpm5 immunoreactivity (red), DAPI (blue) staining was used to visualize nuclei. D – duct of Von Ebner’s gland. Scale bar: 40 μm (Karen K. Yee et al)

Taste cells are located in clusters called taste buds, which in turn are found in papillae, the raised bumps visible on the tongue’s surface. Two types of taste cells contain chemical receptors that initiate perception of sweet, bitter, salty, and sour taste qualities. A third type appears to serve as a supporting cell.

A remarkable characteristic of these sensory cells is that they regularly regenerate. All three taste cell types undergo frequent turnover, with an average lifespan of 10-16 days. As such, new taste cells must constantly be regenerated to replace cells that have died.

For decades, taste scientists have attempted to identify the stem or progenitor cells that spawn the different taste receptor cells. The elusive challenge also sought to establish whether one or several progenitors are involved and where they are located, whether in or near the taste bud.

“Cancer patients who have taste loss following radiation to the head and neck and elderly individuals with diminished taste function are just two populations who could benefit from the ability to activate adult taste stem cells,” said Dr Robert Margolskee of the Monell Chemical Senses Center, who co-authored a paper published in the journal Stem Cells.

Drawing on the strong physiological relationship between oral taste cells and endocrine cells in the intestine, the team used a marker for intestinal stem cells to probe for stem cells in taste tissue on the tongue.

Stains for the stem cell marker, known as leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5), showed two patterns of expression in taste tissue. The first was a strong signal underlying taste papillae at the back of the tongue and the second was a weaker signal immediately underneath taste buds in those papillae.

The scientists hypothesize that the two levels of expression could indicate two different populations of cells. The cells that more strongly express Lgr5 could be true taste stem cells, whereas those with weaker expression could represent those stem cells that have begun the transformation into functional taste cells.

Additional studies revealed that the Lgr5-expressing cells were capable of becoming any one of the three major taste cell types.

“This is just the tip of the iceberg,” said senior author Dr Peihua Jiang, also from the Monell Chemical Senses Center. “Identification of these cells opens up a whole new area for studying taste cell renewal, and contributes to stem cell biology in general.”

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Bibliographic information: Karen K. Yee et al. 2013. Lgr5-EGFP Marks Taste Bud Stem/Progenitor Cells in Posterior Tongue. Stem Cells, accepted for publication; doi: 10.1002/stem.1338