Scientists Identify Gene Required for Nerve Regeneration

A gene that is associated with regeneration of injured nerve cells has been identified by a team of researchers led by Prof Melissa Rolls of Penn State University.

In fruit flies with two normal copies of the spastin gene, a team of scientists led by Prof Melissa Rolls of Penn State University found that severed axons were able to regenerate. However, in fruit flies with two or even only one abnormal spastin gene, the severed axons were not able to regenerate (Melissa Rolls / Penn State University)

The team has found that a mutation in a single gene can entirely shut down the process by which axons – the parts of the nerve cell that are responsible for sending signals to other cells – regrow themselves after being cut or damaged.

“We are hopeful that this discovery will open the door to new research related to spinal-cord and other neurological disorders in humans,” said Prof Rolls, who co-authored a paper published online in the journal Cell Reports.

“Axons, which form long bundles extending out from nerve cells, ideally survive throughout an animal’s lifetime. To be able to survive, nerve cells need to be resilient and, in the event of injury or simple wear and tear, some can repair damage by growing new axons,” Prof Rolls explained.

Previous studies suggested that microtubules – the intracellular ‘highways’ along which basic building blocks are transported – might need to be rebuilt as an important step in this type of repair.

“In many ways this idea makes sense: in order to grow a new part of a nerve, raw materials will be needed, and the microtubule highways will need to be organized to take the new materials to the site of growth,” Prof Rolls said.

The team therefore started to investigate the role of microtubule-remodeling proteins in axon regrowth after injury. In particular, they focused on a set of proteins that sever microtubules into small pieces. Out of this set, a protein named spastin emerged as a key player in axon regeneration.

“The fact that the spastin protein plays a critical role in regeneration is particularly intriguing because, in humans, it is encoded by a disease gene called SPG4,” Prof Rolls said. “When one copy of this gene is disrupted, affected individuals develop hereditary spastic paraplegia (HSP), which is characterized by progressive lower-limb weakness and spasticity as the long-motor axons in the spinal cord degenerate. Thus, identifying a new neuronal function for spastin may help us to understand this disease.”

To study the role of spastin, the team chose the fruit fly as their model organism.

“On the molecular level, many of the processes associated with nerve-cell growth and regrowth are the same in humans as in fruit flies,” Prof Rolls said. “And, like all other animals including humans, fruit flies have two copies of every gene – one from each parent – so different combinations of each gene can lead to different observable traits.”

The team members bred three genetically distinct groups of fruit flies in the laboratory to observe how various spastin gene combinations might affect the behavior of nerve cells after injury. The first group of flies had two normal copies of the gene; the second had one normal copy and one mutant copy; while the third had two mutant copies. Then, in all three groups, the scientists cut the axons of the flies’ nerve cells and observed the regeneration process.

“In fruit flies with two normal copies of the gene, we observed that severed axons elegantly reassembled themselves. This process is supposed to take place if the fly is to heal from nerve trauma since life events, as well as wear and tear, tend to cause such damage,” Prof Rolls said. “But, interestingly, in the other two groups – the fruit flies with two or even one abnormal spastin gene – there was simply no regrowth, indicating that what we have here is a dominant problem.”

The scientists also found that an impaired spastin gene affected only how the axons regrew after being severed. That is, the gene did not seem to play a role in the developmental stage when axons were being assembled for the first time. In addition, the researchers found that, while the gene affected the flies’ axons, their dendrites – the parts of the neuron that receive information from other cells and from the outside world – continued to function and repair themselves normally.

“Now that we know that spastin plays an important role in axon regeneration and also that this gene is dominant, we have opened up a possible path toward the study of human diseases involving nerve-cell impairment,” Prof Rolls said. “In fact, our next step is to probe the link between hereditary spastic paraplegia (HSP) and axon regeneration.”

“The SPG4 gene that encodes human spastin is only one of the disease genes associated with HSP, so we now are testing whether other disease genes also play a role in nerve-cell regeneration,” Prof Rolls said.

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Bibliographic information: Michelle C. Stone et al. Normal Spastin Gene Dosage Is Specifically Required for Axon Regeneration. Cell Reports, 01 November 2012; doi: 10.1016/j.celrep.2012.09.032